ENZYMATIC CLEAVAGE AND REPAIR OF TRANSFORMING DNA
نویسندگان
چکیده
منابع مشابه
Enzymatic Repair of Oxidative DNA Damage1
Oxidative DNA damage, including both mutamente and cytotoxic le sions, is implicated in aging and cancer. Studies of the processes which correct such damage in mammalian cells are, however, still in their early stages. Here we have summarized our recent work which demonstrates new features of mammalian oxidative UNA damage repair, such as (a) a functional role for polylADP-ribosyllation in the ...
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A number of chemotherapeutic agents, such as platinum drugs, nitrogen mustards, and chloroethylnitrosoureas, act by forming bifunctional DNA adducts. It is likely that abortive attempts to replicate and/or repair the damaged DNA cause chromosome aberrations and breakage, leading to cell death. Any substantial increase in cellular capacity to repair damaged DNA may result in resistance to chemot...
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S-adenosylmethionine, known for some time to be the biological methyl donor in mammalian enzymatic transmethylation reactions (1,2), has more recently been implicated in other transalkylation reactions in bacteria and yeast (3-6). The enzyme system catalyzing the condensation of ATP and methionine to form AMe’ has been found in a variety of tissues and organisms, and has been purified from two ...
متن کاملOGG1 DNA Repair Gene Polymorphism As a Biomarker of Oxidative and Genotoxic DNA Damage
Background: Single nucleotide polymorphisms in 8-oxoguanine DNA glycosylase-1 (OGG1) gene modulates DNA repair capacity and functions as one of the first lines of protective mechanisms against 8-hydroxy-2’-deoxyguanosine (8-OHdG) mutagenicity. OGG1-Cys326 gene polymorphism may decrease DNA repair function, causing oxidative stress due to higher oxidative DNA damage. The main purpose of this stu...
متن کاملAn integrated system for enzymatic cleavage and electrostretching of freely-suspended single DNA molecules.
A novel polyacrylamide gel-based femtolitre microchamber system for performing single-molecule restriction enzyme assay on freely-suspended DNA molecules and subsequent DNA electrostretching by applying an alternating electric field has been developed. We attempted the integration by firstly initiating restriction enzyme reaction on a fluorescent-stained lambdaDNA molecule, encapsulated in a mi...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1969
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.62.1.289